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gphn fingr gfp  (Addgene inc)


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    Addgene inc gphn fingr gfp
    (A) Neurospheres were formed by mixing non-electroporated and electroporated cells before being plated in U-bottom ULA wells. (B) Maximum intensity projection image of a confocal stack of a DIV14 neurosphere in which neurons were separately electroporated with RFP (red) and Xph20-GFP (green). (C) Zoomed images on primary dendrites from neurons expressing intrabodies to PSD-95 (Xph20-GFP), or gephyrin <t>(GPHN.FingR-GFP),</t> revealing excitatory or inhibitory post-synapses, respectively. (D) Numbers of PSD-95 and gephyrin-positive puncta per electroporated neuron. Data represent the mean ± SEM of 14 and 10 neurospheres, respectively, and were compared by non-parametric Mann-Whitney test. Dots show individual neurospheres. (E) Maximum intensity projection of a confocal stack of a DIV14 neurosphere in which neurons were co-electroporated with GPHN.FingR-GFP (green) and Xph20-mRuby2 (magenta), allowing the detection of both excitatory and inhibitory post-synapses in the same cells. (F) Zoom on a dendritic segment corresponding to the rectangular area highlighted in (E). (G) Confocal image of a primary dendrite from a neuron expressing GFP-actin, further immunolabeled for GFP, showing numerous dendritic spines bulging out of the shaft.
    Gphn Fingr Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gphn+fingr+gfp/pCAG_GPHN%2EFingR-eGFP-CCR5TC+(Plasmid+%2346296)/bio_rxiv__64898__2026__03__19__712855-158-4-5
    Average 94 stars, based on 29 article reviews
    gphn fingr gfp - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Neurospheres from primary rodent brain cells to probe the 3D organization and function of synapses"

    Article Title: Neurospheres from primary rodent brain cells to probe the 3D organization and function of synapses

    Journal: bioRxiv

    doi: 10.64898/2026.03.19.712855

    (A) Neurospheres were formed by mixing non-electroporated and electroporated cells before being plated in U-bottom ULA wells. (B) Maximum intensity projection image of a confocal stack of a DIV14 neurosphere in which neurons were separately electroporated with RFP (red) and Xph20-GFP (green). (C) Zoomed images on primary dendrites from neurons expressing intrabodies to PSD-95 (Xph20-GFP), or gephyrin (GPHN.FingR-GFP), revealing excitatory or inhibitory post-synapses, respectively. (D) Numbers of PSD-95 and gephyrin-positive puncta per electroporated neuron. Data represent the mean ± SEM of 14 and 10 neurospheres, respectively, and were compared by non-parametric Mann-Whitney test. Dots show individual neurospheres. (E) Maximum intensity projection of a confocal stack of a DIV14 neurosphere in which neurons were co-electroporated with GPHN.FingR-GFP (green) and Xph20-mRuby2 (magenta), allowing the detection of both excitatory and inhibitory post-synapses in the same cells. (F) Zoom on a dendritic segment corresponding to the rectangular area highlighted in (E). (G) Confocal image of a primary dendrite from a neuron expressing GFP-actin, further immunolabeled for GFP, showing numerous dendritic spines bulging out of the shaft.
    Figure Legend Snippet: (A) Neurospheres were formed by mixing non-electroporated and electroporated cells before being plated in U-bottom ULA wells. (B) Maximum intensity projection image of a confocal stack of a DIV14 neurosphere in which neurons were separately electroporated with RFP (red) and Xph20-GFP (green). (C) Zoomed images on primary dendrites from neurons expressing intrabodies to PSD-95 (Xph20-GFP), or gephyrin (GPHN.FingR-GFP), revealing excitatory or inhibitory post-synapses, respectively. (D) Numbers of PSD-95 and gephyrin-positive puncta per electroporated neuron. Data represent the mean ± SEM of 14 and 10 neurospheres, respectively, and were compared by non-parametric Mann-Whitney test. Dots show individual neurospheres. (E) Maximum intensity projection of a confocal stack of a DIV14 neurosphere in which neurons were co-electroporated with GPHN.FingR-GFP (green) and Xph20-mRuby2 (magenta), allowing the detection of both excitatory and inhibitory post-synapses in the same cells. (F) Zoom on a dendritic segment corresponding to the rectangular area highlighted in (E). (G) Confocal image of a primary dendrite from a neuron expressing GFP-actin, further immunolabeled for GFP, showing numerous dendritic spines bulging out of the shaft.

    Techniques Used: Expressing, MANN-WHITNEY, Immunolabeling

    Related Articles

    Expressing:

    Article Title: Neurospheres from primary rodent brain cells to probe the 3D organization and function of synapses
    Article Snippet: The intrabody to PSD-95, Xph20-GFP (Addgene #135,530 pCAG_Xph20-eGFP-CCR5TC), was a gift from M. Sainlos (IINS, University of Bordeaux) ( ).The intrabody to PSD-95, Xph20-GFP (Addgene #135,530 pCAG_Xph20-eGFP-CCR5TC), was a gift from M. Sainlos (IINS, University of Bordeaux) ( ).. The intrabody to gephyrin, GPHN.FingR-GFP (Addgene # 46296 pCAG_GPHN.FingR-eGFP-CCR5TC), was a gift from D. Arnold (University of Southern California, Los Angeles, CA, USA) ( ).. Short hairpin RNA to murine NLGN1, and its control shRNA to the protein p53 , were gifts from P. Scheiffele (Biozentrum, Basel, Switzerland).Short hairpin RNA to murine NLGN1, and its control shRNA to the protein p53 , were gifts from P. Scheiffele (Biozentrum, Basel, Switzerland).

    Article Title: High-affinity detection of endogenously biotinylated neuroligin-1 at excitatory and inhibitory synapses using a tagged knock-in mouse strain
    Article Snippet: (Addgene #135,530 pCAG Xph20-eGFP-CCR5TC; #135,531 pCAG Xph20-mRuby2- CCR5TC), were gifts from M. Sainlos (IINS, University of Bordeaux). .. The intrabody to gephyrin, GPHN.FingR-GFP , (Addgene # 46296 pCAG_GPHN.FingR-eGFP-CCR5TC), was a gift from D. Arnold (University of Southern California, Los Angeles, CA, USA). .. Short hairpin RNA to murine NLGN1, and its control shRNA to the heat shock protein p53 , were kind gifts from P. Scheiffele (Biozentrum, Basel, Switze

    MANN-WHITNEY:

    Article Title: Neurospheres from primary rodent brain cells to probe the 3D organization and function of synapses
    Article Snippet: The intrabody to PSD-95, Xph20-GFP (Addgene #135,530 pCAG_Xph20-eGFP-CCR5TC), was a gift from M. Sainlos (IINS, University of Bordeaux) ( ).The intrabody to PSD-95, Xph20-GFP (Addgene #135,530 pCAG_Xph20-eGFP-CCR5TC), was a gift from M. Sainlos (IINS, University of Bordeaux) ( ).. The intrabody to gephyrin, GPHN.FingR-GFP (Addgene # 46296 pCAG_GPHN.FingR-eGFP-CCR5TC), was a gift from D. Arnold (University of Southern California, Los Angeles, CA, USA) ( ).. Short hairpin RNA to murine NLGN1, and its control shRNA to the protein p53 , were gifts from P. Scheiffele (Biozentrum, Basel, Switzerland).Short hairpin RNA to murine NLGN1, and its control shRNA to the protein p53 , were gifts from P. Scheiffele (Biozentrum, Basel, Switzerland).

    Article Title: High-affinity detection of endogenously biotinylated neuroligin-1 at excitatory and inhibitory synapses using a tagged knock-in mouse strain
    Article Snippet: (Addgene #135,530 pCAG Xph20-eGFP-CCR5TC; #135,531 pCAG Xph20-mRuby2- CCR5TC), were gifts from M. Sainlos (IINS, University of Bordeaux). .. The intrabody to gephyrin, GPHN.FingR-GFP , (Addgene # 46296 pCAG_GPHN.FingR-eGFP-CCR5TC), was a gift from D. Arnold (University of Southern California, Los Angeles, CA, USA). .. Short hairpin RNA to murine NLGN1, and its control shRNA to the heat shock protein p53 , were kind gifts from P. Scheiffele (Biozentrum, Basel, Switze

    Immunolabeling:

    Article Title: Neurospheres from primary rodent brain cells to probe the 3D organization and function of synapses
    Article Snippet: The intrabody to PSD-95, Xph20-GFP (Addgene #135,530 pCAG_Xph20-eGFP-CCR5TC), was a gift from M. Sainlos (IINS, University of Bordeaux) ( ).The intrabody to PSD-95, Xph20-GFP (Addgene #135,530 pCAG_Xph20-eGFP-CCR5TC), was a gift from M. Sainlos (IINS, University of Bordeaux) ( ).. The intrabody to gephyrin, GPHN.FingR-GFP (Addgene # 46296 pCAG_GPHN.FingR-eGFP-CCR5TC), was a gift from D. Arnold (University of Southern California, Los Angeles, CA, USA) ( ).. Short hairpin RNA to murine NLGN1, and its control shRNA to the protein p53 , were gifts from P. Scheiffele (Biozentrum, Basel, Switzerland).Short hairpin RNA to murine NLGN1, and its control shRNA to the protein p53 , were gifts from P. Scheiffele (Biozentrum, Basel, Switzerland).

    Article Title: High-affinity detection of endogenously biotinylated neuroligin-1 at excitatory and inhibitory synapses using a tagged knock-in mouse strain
    Article Snippet: (Addgene #135,530 pCAG Xph20-eGFP-CCR5TC; #135,531 pCAG Xph20-mRuby2- CCR5TC), were gifts from M. Sainlos (IINS, University of Bordeaux). .. The intrabody to gephyrin, GPHN.FingR-GFP , (Addgene # 46296 pCAG_GPHN.FingR-eGFP-CCR5TC), was a gift from D. Arnold (University of Southern California, Los Angeles, CA, USA). .. Short hairpin RNA to murine NLGN1, and its control shRNA to the heat shock protein p53 , were kind gifts from P. Scheiffele (Biozentrum, Basel, Switze

    Fluorescence:

    Article Title: Neurospheres from primary rodent brain cells to probe the 3D organization and function of synapses
    Article Snippet: The intrabody to PSD-95, Xph20-GFP (Addgene #135,530 pCAG_Xph20-eGFP-CCR5TC), was a gift from M. Sainlos (IINS, University of Bordeaux) ( ).The intrabody to PSD-95, Xph20-GFP (Addgene #135,530 pCAG_Xph20-eGFP-CCR5TC), was a gift from M. Sainlos (IINS, University of Bordeaux) ( ).. The intrabody to gephyrin, GPHN.FingR-GFP (Addgene # 46296 pCAG_GPHN.FingR-eGFP-CCR5TC), was a gift from D. Arnold (University of Southern California, Los Angeles, CA, USA) ( ).. Short hairpin RNA to murine NLGN1, and its control shRNA to the protein p53 , were gifts from P. Scheiffele (Biozentrum, Basel, Switzerland).Short hairpin RNA to murine NLGN1, and its control shRNA to the protein p53 , were gifts from P. Scheiffele (Biozentrum, Basel, Switzerland).

    Article Title: High-affinity detection of endogenously biotinylated neuroligin-1 at excitatory and inhibitory synapses using a tagged knock-in mouse strain
    Article Snippet: (Addgene #135,530 pCAG Xph20-eGFP-CCR5TC; #135,531 pCAG Xph20-mRuby2- CCR5TC), were gifts from M. Sainlos (IINS, University of Bordeaux). .. The intrabody to gephyrin, GPHN.FingR-GFP , (Addgene # 46296 pCAG_GPHN.FingR-eGFP-CCR5TC), was a gift from D. Arnold (University of Southern California, Los Angeles, CA, USA). .. Short hairpin RNA to murine NLGN1, and its control shRNA to the heat shock protein p53 , were kind gifts from P. Scheiffele (Biozentrum, Basel, Switze



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    Image Search Results


    (A) Neurospheres were formed by mixing non-electroporated and electroporated cells before being plated in U-bottom ULA wells. (B) Maximum intensity projection image of a confocal stack of a DIV14 neurosphere in which neurons were separately electroporated with RFP (red) and Xph20-GFP (green). (C) Zoomed images on primary dendrites from neurons expressing intrabodies to PSD-95 (Xph20-GFP), or gephyrin (GPHN.FingR-GFP), revealing excitatory or inhibitory post-synapses, respectively. (D) Numbers of PSD-95 and gephyrin-positive puncta per electroporated neuron. Data represent the mean ± SEM of 14 and 10 neurospheres, respectively, and were compared by non-parametric Mann-Whitney test. Dots show individual neurospheres. (E) Maximum intensity projection of a confocal stack of a DIV14 neurosphere in which neurons were co-electroporated with GPHN.FingR-GFP (green) and Xph20-mRuby2 (magenta), allowing the detection of both excitatory and inhibitory post-synapses in the same cells. (F) Zoom on a dendritic segment corresponding to the rectangular area highlighted in (E). (G) Confocal image of a primary dendrite from a neuron expressing GFP-actin, further immunolabeled for GFP, showing numerous dendritic spines bulging out of the shaft.

    Journal: bioRxiv

    Article Title: Neurospheres from primary rodent brain cells to probe the 3D organization and function of synapses

    doi: 10.64898/2026.03.19.712855

    Figure Lengend Snippet: (A) Neurospheres were formed by mixing non-electroporated and electroporated cells before being plated in U-bottom ULA wells. (B) Maximum intensity projection image of a confocal stack of a DIV14 neurosphere in which neurons were separately electroporated with RFP (red) and Xph20-GFP (green). (C) Zoomed images on primary dendrites from neurons expressing intrabodies to PSD-95 (Xph20-GFP), or gephyrin (GPHN.FingR-GFP), revealing excitatory or inhibitory post-synapses, respectively. (D) Numbers of PSD-95 and gephyrin-positive puncta per electroporated neuron. Data represent the mean ± SEM of 14 and 10 neurospheres, respectively, and were compared by non-parametric Mann-Whitney test. Dots show individual neurospheres. (E) Maximum intensity projection of a confocal stack of a DIV14 neurosphere in which neurons were co-electroporated with GPHN.FingR-GFP (green) and Xph20-mRuby2 (magenta), allowing the detection of both excitatory and inhibitory post-synapses in the same cells. (F) Zoom on a dendritic segment corresponding to the rectangular area highlighted in (E). (G) Confocal image of a primary dendrite from a neuron expressing GFP-actin, further immunolabeled for GFP, showing numerous dendritic spines bulging out of the shaft.

    Article Snippet: The intrabody to gephyrin, GPHN.FingR-GFP (Addgene # 46296 pCAG_GPHN.FingR-eGFP-CCR5TC), was a gift from D. Arnold (University of Southern California, Los Angeles, CA, USA) ( ).

    Techniques: Expressing, MANN-WHITNEY, Immunolabeling